Abstract:
Common methods of synthesizing metallic nanoparticles are chemical and physical. However, they
are expensive and use toxic chemicals. Green synthesis is less costly and safer, hence a potential
alternative. Silver nanoparticles (Ag NPs) were synthesized using dichloromethane extract of
Chrysanthemum cinerariaefolium, and colour change from pale green to dark brown was observed.
Scanning electron microscopy (SEM) images were faceted, and others formed clusters. Transmission
electron microscopy (TEM) images were spherical with an average size of 22.8± 17.5 nm. EDX
analysis showed the nanoparticles had percentage abundance of 67.26%. Fourier-transform infrared
spectroscopy (FTIR) analysis showed absorption bands at 3489.59 cm-1, 3217.80 cm-1, 2384.74 cm-1,
1633.05 cm-1, 1405.08 cm-1, 1109.32 cm-1, and 505.93 cm-1. The UV-Vis analysis showed surface
plasmon resonance (SPR) peak at 434 nm. The nanoparticles were more active on P. aeruginosa with
an MIC of 15 µg/ml while the cytotoxicity assay showed Ag NPs had an MIC of 33.33 µg/ml, and
hence, were noncytotoxic against Vero cells
