Abstract:
Immunocytochemistry and cell viability imaging by labeling techniques
permit studying spatial and temporal dynamics of virusspreading on host
cell surfaces in vitro.
Haseltine et al. [1] developed an imaging method that permits tracking
the spread of focal viral infection using a combination of
immunocytochemical labeling and step-by-step digital imaging.
Baby hamster kidney (BHK) cells were seeded on six well plates,
grown as confluent monolayers and covered with a thin layer of agar.
After piercing a small orifice in the agar, cell layers were infected by
injecting 5 μl of virus VSVN1 inoculum with 1.6x107 infectious
particles (a multiplicity of infection of 20). Cells were subsequently
fixed, and immunofluorescence labeled with an antibody against a viral
glycoprotein on and within the infected cells.
