dc.contributor.author |
Inder Sehgal |
|
dc.contributor.author |
Hairong Li |
|
dc.contributor.author |
Ongarora, Benson G. |
|
dc.contributor.author |
Daniel Devillier |
|
dc.contributor.author |
M. Graça H. Vicente |
|
dc.date.accessioned |
2021-09-09T07:50:52Z |
|
dc.date.available |
2021-09-09T07:50:52Z |
|
dc.date.issued |
2013-01 |
|
dc.identifier.uri |
doi:10.1142/S108842461250143X |
|
dc.identifier.uri |
http://repository.dkut.ac.ke:8080/xmlui/handle/123456789/4858 |
|
dc.description.abstract |
Two zinc(II) phthalocyanines (ZnPcs) were conjugated with a monoclonal antibody (MAb)
directed against carcinoembryonic antigen (CEA), using an in situ activated carboxylic acid on the
ZnPcs. The bioconjugate with the highest ZnPc/MAb ratio of 3 was investigated in vitro for its
ability to target and fluorescently label human colorectal HT-29 cells. The ZnPc-CEA MAb 2 was
observed to efficiently target HT-29 cells, about 37 times more than unconjugated ZnPc.
Furthermore, in the presence of a 4-fold excess of unlabelled anti-CEA antibody, the fluorescence
signal of 2 was reduced by ~90% showing that the targeting is CEA-mediated. These studies
further confirm the high specificity of Pc-antibody conjugates for antigens over-expressed on
tumor cells and warrant further investigations of these immunoconjugates and their derivatives for
imaging of colorectal cancer. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
World Scientific Publishing Company |
en_US |
dc.title |
Synthesis and biological investigations of a ZnPc-antiCEA bioconjugate for imaging of colorectal cancer |
en_US |
dc.type |
Article |
en_US |