Quantitative E. coli Enzyme Detection in Reporter Hydrogel-Coated Paper Using a Smartphone Camera

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dc.contributor.author Karuri, Nancy Wangechi
dc.contributor.author Holger Schönherr
dc.contributor.author Mareike Müller
dc.contributor.author Sergey I. Druzhinin
dc.contributor.author Winny Chelangat
dc.contributor.author Kawaljit Kaur
dc.contributor.author Sergey I. Druzhinin
dc.date.accessioned 2021-02-05T09:41:15Z
dc.date.available 2021-02-05T09:41:15Z
dc.date.issued 2021-01
dc.identifier.other https://doi.org/10.3390/bios11010025
dc.identifier.uri http://repository.dkut.ac.ke:8080/xmlui/handle/123456789/4674
dc.description.abstract There is a growing demand for rapid and sensitive detection approaches for pathogenic bacteria that can be applied by non-specialists in non-laboratory field settings. Here, the detection of the typical E. coli enzyme β-glucuronidase using a chitosan-based sensing hydrogel-coated paper sensor and the detailed analysis of the reaction kinetics, as detected by a smartphone camera, is reported. The chromogenic reporter unit affords an intense blue color in a two-step reaction, which was analyzed using a modified Michaelis–Menten approach. This generalizable approach can be used to determine the limit of detection and comprises an invaluable tool to characterize the performance of lab-in-a-phone type approaches. For the particular system analyzed, the ratio of reaction rate and equilibrium constants of the enzyme–substrate complex are 0.3 and 0.9 pM−1h −1 for β-glucuronidase in phosphate buffered saline and lysogeny broth, respectively. The minimal degree of substrate conversion for detection of the indigo pigment formed during the reaction is 0.15, while the minimal time required for detection in this particular system is ~2 h at an enzyme concentration of 100 nM. Therefore, this approach is applicable for quantitative lab-in-a-phone based point of care detection systems that are based on enzymatic substrate conversion via bacterial enzymes en_US
dc.language.iso en en_US
dc.publisher MDPI en_US
dc.title Quantitative E. coli Enzyme Detection in Reporter Hydrogel-Coated Paper Using a Smartphone Camera en_US
dc.type Article en_US

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